Genetic diversity and population structure of the Chilean native, Tillandsia landbeckii (Bromeliaceae), from the Atacama Desert

Elizabeth Bastías, Edith Choque-Ayaviri, Joel Flores, Glenda Fuentes-Arce, Patricio López-Sepúlveda, Wilson Huanca-Mamani


Tillandsia landbeckii Phil. is a typical plant of the Atacama Desert in the north of Chile. There is no genetic data available at the population level for this species, and this information is critical for developing and implementing effective conservation measures. In this study, we investigated for the first time the genetic diversity and population 
structure in 2 natural populations of T. landbeckii using AFLP markers. Seven primer combinations produced 405 bands and of them, 188 (46.42%) were polymorphic. The Pampa Dos Cruces population (Pp = 88.30%, He = 0.327, and I = 0.483) showed a higher genetic diversity level than Pampa Camarones population (Pp = 71.28%, He = 0.253,
and I = 0.380). Analysis of molecular variance (Amova) revealed that 25.12% of the total genetic diversity resided among populations, while 74.88% within populations. A moderate-high genetic differentiation coefficient (Fst = 0.251) and a moderate population gene flow (Nm = 1.490) were also observed. Principal coordinates analysis (PCoA), 
Neighbor-net and STRUCTURE analysis supported the grouping of the sampled populations into 2 moderate genetic clusters. This first study provides data that will allow assist and support conservation decisions taken for this species.

Palabras clave

Molecular markers; AFLP; Tillandsia landbeckii

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Barfuss, M. H. J., Samuel, R., Till, W., & Stuessy, T. F. (2005). Phylogenetic relationships in subfamily Tillandsioideae (Bromeliaceae) based on DNA sequence data from seven plastid regions. American Journal of Botany, 92(2), 337–351.

Cascante-Marín, A., Oostermeijer, G., Wolf, J., & Fuchs, E. J. (2014). Genetic Diversity and Spatial Genetic Structure of an Epiphytic Bromeliad in Costa Rican Montane Secondary Forest Patches. Biotropica, 46(4), 425–432.

Earl, D. A., & von Holdt, B. M. (2011). STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources, 4(2), 359–361.

EL-Bakatoushi, R., & Ahmed, D. G. A. (2018). Evaluation of genetic diversity in wild populations of Peganum harmala L., a medicinal plant. Journal of Genetic Engineering and Biotechnology, 16(1), 143–151.

Evanno, G., Regnaut, S., & Goudet, J. (2005). Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology, 14(8), 2611–2620.

Excoffier, L. & Lischer H. E. L. (2010). Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10, 564–567.

Frankham, R. (2003). Genetics and conservation biology. Comptes Rendus Biologies, 326 Suppl 1, S22–S29.

Frankham, R., Ballou, J. D., & Briscoe, D. A. (2010). Introduction to Conservation Genetics. Second Edition. Cambridge University Press.

González-Astorga, J., Cruz-Angón, A., Flores-Palacios, A., & Vovides, A. P. (2004). Diversity and genetic structure of the Mexican endemic epiphyte Tillandsia achyrostachys E. Morr. ex Baker var. achyrostachys (Bromeliaceae). Annals of Botany, 94(4), 545–551.

Gordon, S. P., Sloop, C. M., Davis, H. G., & Hall Cushman, J. (2011). Population genetic diversity and structure of two rare vernal pool grasses in central California. Conservation Genetics, 13(1), 117–130.

Hamrick, J.L. & Godt, M. J. W. (1996). Effects of life history traits on genetic diversity in plant species. Philosophical Transactions of the Royal Society London [Biological Sciences], 351, 1291–1298.

Hamrick, J. L., & Nason, J. D. (2000). Gene flow in forest trees. In A. Young, D. Boshier & T. Boyle (Eds), Forest Conservation Genetics: Principles and Practice (pp. 81–90). CSIRO Publishing, Collingwood, Australia.

Hartl, D. L., & Clark, A. G. (1997). Principles of Population Genetics. Third Edition. Sinauer Associates Incorporated. Massachusetts, USA.

Holsinger, K. E., & Gottlieb, L. D. (1989). The conservation of rare and endangered plants. Trends in Ecology & Evolution, 4(7), 193–194.

Huanca-Mamani, W., Rivera-Cabello, D., & Maita-Maita, J. (2015). A simple, fast, and inexpensive CTAB-PVP-silica based method for genomic DNA isolation from single, small insect larvae and pupae. Genetics and Molecular Research, 14, 8001–8007.

Huson, D. H., & Bryant, D. (2006). Application of phylogenetic networks in evolutionary studies. Molecular Biology and Evolution, 23(2), 254–267.

Jiménez, A., Weigelt, B., Santos-Guerra, A., Caujapé-Castells, J., Fernández-Palacios, J. M., & Conti, E. (2017). Surviving in isolation: genetic variation, bottlenecks and reproductive strategies in the Canarian endemic Limonium macrophyllum (Plumbaginaceae). Genetica, 145(1), 91–104.

Neel, M. C., & Ellstrand, N. C. (2001). Patterns of allozyme diversity in the threatened plant Erigeron parishii (Asteraceae). American Journal of Botany, 88(5), 810–818.

Neri, J., Wendt, T., & Palma-Silva, C. (2018). Natural hybridization and genetic and morphological variation between two epiphytic bromeliads. AoB Plants, 10(1), plx061.

Nybom, H. (2004). Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology, 13(5), 1143–1155.

Peakall, R., & Smouse, P. E. (2012). GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research an update. Bioinformatics, 28(19), 2537–2539.

Peng, Y. Q., Fan, L. L., Mao, F. Y., Zhao, Y. S., Xu, R., Yin, Y. J., … Zhang, X. H. (2018). Genetic diversity and population structure of a protected species: Polygala tenuifolia Willd. Comptes Rendus Biologies, 341(3), 152–159.

Pinto, R. (2005). Tillandsia del Norte de Chile y del extremo Sur de Perú. A. Molina Flores, Santiago.

Pinto, R., Barría, I., & Marquet, P. A. (2006). Geographical distribution of Tillandsia lomas in the Atacama Desert, northern Chile. Journal of Arid Environments, 65(4), 543–552.

Pritchard, J. K., Stephens, M., & Donnelly, P. (2000). Inference of population structure using multilocus genotype data. Genetics, 155(2), 945–959.

Rodriguez, R., Marticorena, C., Alarcón, D., Baeza, C., Cavieres, L., Finot, V. L., … Marticorena, A. (2018). Catálogo de las plantas vasculares de Chile. Gayana Botánica, 75(1), 1–430.

Rundel, P. W., & Dillon, M. O. (1998). Ecological patterns in the Bromeliaceae of the lomas formations of Coastal Chile and Peru. Plant Systematics and Evolution = Entwicklungsgeschichte Und Systematik Der Pflanzen, 212(3-4), 261–278.

Rundel, P.W., Palma B., Dillon, M.O., Sharifi, M.R. & Boonpragob, K. (1997). Tillandsia landbeckii in the coastal Atacama Desert of northern Chile. Revista Chilena de Historia Natural, 70, 341–349.

Sarricolea, P., Ruiz, O. M., & Romero-Aravena, H. (2017). Tendencias de la precipitación en el norte grande de Chile y su relación con las proyecciones de cambio climático. Diálogo Andino, (54), 41–50.

Sheidai, M., Afshar, F., Keshavarzi, M., Talebi, S.-M., Noormohammadi, Z., & Shafaf, T. (2014). Genetic diversity and genome size variability in Linum austriacum (Lineaceae) populations. Biochemical Systematics and Ecology, 57, 20–26.

Soares, L. E., Goetze, M., Zanella, C. M., & Bered, F. (2018). Genetic diversity and population structure of Vriesea reitzii (Bromeliaceae), a species from the Southern Brazilian Highlands. Genetics and Molecular Biology, 41(1 suppl 1), 308–317.

Solórzano, S., Solís, S. J. & Dávila, P. (2010). Low Genetic Diversity in Tillandsia recurvata (Bromeliaceae), the Most Ubiquitous Epiphyte Species of the Semiarid and Arid Zones of North America. Journal of the Bromeliad Society, 60(2), 71–81.

Soltis, D. E., Gilmartin, A. J., Rieseberg, L., & Gardner, S. (1987). Genetic Variation in the Epiphytes Tillandsia ionantha and T. recurvata (Bromeliaceae). American Journal of Botany, 74(4), 531.

Tansley, S. A., & Brown, C. R. (2000). RAPD variation in the rare and endangered Leucadendron elimense (Proteaceae): implications for their conservation. Biological Conservation, 95(1), 39–48.

Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., … Zabeau, M. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23(21), 4407–4414.

Wright, S. (1969). Evolution and genetics of populations vol 2. The theory of gene frequencies. University of Chicago Press, Chicago

Zhang, C., Zhang, J., Fan, Y., Sun, M., Wu, W., Zhao, W., … Zhang, X. (2017). Genetic Structure and Eco-Geographical Differentiation of Wild Sheep Fescue (Festuca ovina L.) in Xinjiang, Northwest China. Molecules, 22(8), 1316.

Zizka, G., Schmidt, M., Schulte, K., Novoa, P., Pinto, R., & König, K. (2009). Chilean Bromeliaceae: diversity, distribution and evaluation of conservation status. Biodiversity and Conservation, 18(9), 2449–2471.


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Revista Mexicana de Biodiversidad, Año 3, No. 12, enero-marzo 2014, es una publicación trimestral editada por la Universidad Nacional Autónoma de México, Ciudad Universitaria, Delegación Coyoacán, C.P. 04510, Ciudad de México, a través del Instituto de Biología, Tercer Circuito Universitario s/n, Ciudad Universitaria, Col. Copilco, Del. Coyoacán, C.P. 04510, Ciudad de México, Tel. (55)56229164,, responsable: Dr. Fernando Álvarez Noguera. Reserva de Derechos al uso Exclusivo No. 04-2013-092709142100-203, ISSN: 2007-8706, ambos otorgados por el Instituto Nacional del Derecho de Autor, Responsable de la última actualización de este número, Instituto de Biología, UNAM, Dr. Fernando Álvarez Noguera, Tercer Circuito Universitario s/n, Ciudad Universitaria, Col. Copilco, Del. Coyoacán, C.P. 04510, Ciudad de México, fecha de la última modificación: 25 de agosto de 2016.

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